goat anti protamine 2 (Santa Cruz Biotechnology)
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goat anti protamine 2
Goat Anti Protamine 2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 12093 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protamine+2/goat+anti-rabbit+IgG-B/pmc11075221-386-30-34
Average 96 stars, based on 12093 article reviews
Goat Anti Protamine 2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 12093 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protamine+2/goat+anti-rabbit+IgG-B/pmc11075221-386-30-34
Average 96 stars, based on 12093 article reviews
goat anti protamine 2 - by Bioz Stars,
2026-10
96/100 stars
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Staining:Article Title: Cryopreservation of adult cervid testes. Article Snippet: Several species of cervids are currently classified as threatened or endangered due to a rapid decline in their populations.. Sperm cryopreservation, in association with assisted reproductive technologies, can find application for the conservation of endangered cervids.. In cases of unsuccessful sperm retrieval through other means prior to the death of the animal, adult testis is the only source of sperm. Expressing:Article Title: Cryopreservation of adult cervid testes. Article Snippet: Several species of cervids are currently classified as threatened or endangered due to a rapid decline in their populations.. Sperm cryopreservation, in association with assisted reproductive technologies, can find application for the conservation of endangered cervids.. In cases of unsuccessful sperm retrieval through other means prior to the death of the animal, adult testis is the only source of sperm. Western Blot:Article Title: Changes in vinexin expression patterns in the mouse testis induced by developmental exposure to 17beta-estradiol. Article Snippet: Total RNA was extracted from testes at different developmental stages (8, 12, 18, 30, and 120 dpn), from isolated testicular cells, and from somatic tissues using TRIzol (Invitrogen) according to the manufacturer’s instructions. .. Antibodies For immunoblot analyses, the following primary antibodies were used: 1) affinity-purified rabbit polyclonal antibodies raised against vinexin (kindly provided by Dr. Kioka, [9]); 2) goat polyclonal antibodies raised against Affinity Purification:Article Title: Changes in vinexin expression patterns in the mouse testis induced by developmental exposure to 17beta-estradiol. Article Snippet: Total RNA was extracted from testes at different developmental stages (8, 12, 18, 30, and 120 dpn), from isolated testicular cells, and from somatic tissues using TRIzol (Invitrogen) according to the manufacturer’s instructions. .. Antibodies For immunoblot analyses, the following primary antibodies were used: 1) affinity-purified rabbit polyclonal antibodies raised against vinexin (kindly provided by Dr. Kioka, [9]); 2) goat polyclonal antibodies raised against Bioprocessing:Article Title: Changes in vinexin expression patterns in the mouse testis induced by developmental exposure to 17beta-estradiol. Article Snippet: Total RNA was extracted from testes at different developmental stages (8, 12, 18, 30, and 120 dpn), from isolated testicular cells, and from somatic tissues using TRIzol (Invitrogen) according to the manufacturer’s instructions. .. Antibodies For immunoblot analyses, the following primary antibodies were used: 1) affinity-purified rabbit polyclonal antibodies raised against vinexin (kindly provided by Dr. Kioka, [9]); 2) goat polyclonal antibodies raised against Gel Extraction:Article Title: Autoimmune Regulator (AIRE) Is Expressed in Spermatogenic Cells, and It Altered the Expression of Several Nucleic-Acid-Binding and Cytoskeletal Proteins in Germ Cell 1 Spermatogonial (GC1-spg) Cells Article Snippet: .. Lipofectamine 2000, DMEM, DMEMF12, OptiMEM, FBS, Antibiotic-antimycotic cocktail LGlutamine, 1X MEM vitamins, Nonessential amino acids (Invitrogen, Green Island, CA), StemPro34 SFM base (Invitrogen, Green Island, CA); EcoR1, Sal1, CIP, NEB buffer 3, EcoR1 Buffer, DNA Ligase and Ligase buffer (New England Biolabs, Ipswich, MA); Nucleobond Xtra midi Plus EF kit (Macherey Nagel, Duren, Germany); Gel extraction kit (GE Healthcare, Buckinghamshire, UK); 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), MgCl2, KCl, NaCl, 2,2',2'',2'''-(Ethane-1,2-diyldinitrilo) tetra acetic acid (EDTA), Glycerol, 6 SUPERSCRIPT ® VILO TM cDNA synthesis kit (Invitrogen, Waltham, MA); SYBR green master mix plates, ABI PRISM ® 384 well optical reaction (Applied Bio systems, Waltham, MA), QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany); Protease inhibitor cocktail, Bovine serum albumin (Calbiochem, San Diego, CA); TRI reagent, Glucose, d-Biotin, Insulin, Pyruvic acid sodium salt, dl-Lactic acid, Ascorbic acid, Sodium selenite, Putrescine, Bovine Apotransferrin, Progesterone, β-Estradiol 17-cypionate, 2-Mercaptoethanol (Sigma- Aldrich, Carlsbad, MO); Fetal bovine serum (Hyclone, Logan, UT); SCP-3, Histone H3 (Abcam, Cambridge, United Kingdom); anti-Aire antibody (sc-33188), AIRE-1 (M-300), GFRα-1 (H-70), OCT-3/4 (H-134), Article Title: Autoimmune Regulator ( AIRE ) Is Expressed in Spermatogenic Cells, and It Altered the Expression of Several Nucleic-Acid-Binding and Cytoskeletal Proteins in Germ Cell 1 Spermatogonial (GC1-spg) Cells Article Snippet: .. Reagents Lipofectamine 2000, DMEM, DMEMF12, OptiMEM, FBS, antibiotic–antimycotic mixture l -glutamine, 1X MEM vitamins, nonessential amino acids StemPro34 SFM base (Invitrogen, Green Island, CA); EcoR1, Streptomyces albus 1 (Sal1), calf intestinal phosphatase (CIP), New England Biolab (NEB) buffer 3, EcoR1 Buffer, DNA ligase, and ligase buffer (New England Biolabs, Ipswich, MA); Nucleobond Xtra midi Plus EF kit (Macherey Nagel, Duren, Germany); gel extraction kit (GE Healthcare, Buckinghamshire, UK); 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), MgCl 2 , KCl, NaCl, 2,2′,2″,2‴-(ethane-1,2-diyldinitrilo) tetra acetic acid (EDTA), glycerol, SUPERSCRIPT ® VILO TM cDNA synthesis kit (Invitrogen, Waltham, MA); SYBR green master mix plates, ABI PRISM ® 384-well optical reaction (Applied Bio systems, Waltham, MA), QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany); protease inhibitor mixture, bovine serum albumin (Calbiochem, San Diego, CA); Total RNA isolation (TRI) reagent, glucose, d-biotin, insulin, pyruvic acid sodium salt, dl-lactic acid, ascorbic acid, sodium selenite, putrescine, bovine Apo-transferrin, progesterone, β-estradiol 17-cypionate, 2-mercaptoethanol (Sigma- Aldrich, Carlsbad, MO); fetal bovine serum (Hyclone, Logan, UT); SCP-3, histone H3 (Abcam, Cambridge, United Kingdom); anti-Aire antibody (sc-33188), AIRE-1 (M-300), GFRα-1 (H-70), OCT-3/4 (H-134), Article Title: Autoimmune Regulator ( AIRE ) Is Expressed in Spermatogenic Cells, and It Altered the Expression of Several Nucleic-Acid-Binding and Cytoskeletal Proteins in Germ Cell 1 Spermatogonial (GC1-spg) Cells Article Snippet: .. Lipofectamine 2000, DMEM, DMEMF12, OptiMEM, FBS, antibiotic–antimycotic mixture l -glutamine, 1X MEM vitamins, nonessential amino acids StemPro34 SFM base (Invitrogen, Green Island, CA); EcoR1, Streptomyces albus 1 (Sal1), calf intestinal phosphatase (CIP), New England Biolab (NEB) buffer 3, EcoR1 Buffer, DNA ligase, and ligase buffer (New England Biolabs, Ipswich, MA); Nucleobond Xtra midi Plus EF kit (Macherey Nagel, Duren, Germany); gel extraction kit (GE Healthcare, Buckinghamshire, UK); 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), MgCl 2 , KCl, NaCl, 2,2′,2′′,2′′′-(ethane-1,2-diyldinitrilo) tetra acetic acid (EDTA), glycerol, SUPERSCRIPT ® VILO TM cDNA synthesis kit (Invitrogen, Waltham, MA); SYBR green master mix plates, ABI PRISM ® 384-well optical reaction (Applied Bio systems, Waltham, MA), QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany); protease inhibitor mixture, bovine serum albumin (Calbiochem, San Diego, CA); Total RNA isolation (TRI) reagent, glucose, d-biotin, insulin, pyruvic acid sodium salt, dl-lactic acid, ascorbic acid, sodium selenite, putrescine, bovine Apo-transferrin, progesterone, β-estradiol 17-cypionate, 2-mercaptoethanol (Sigma- Aldrich, Carlsbad, MO); fetal bovine serum (Hyclone, Logan, UT); SCP-3, histone H3 (Abcam, Cambridge, United Kingdom); anti-Aire antibody (sc-33188), AIRE-1 (M-300), GFRα-1 (H-70), OCT-3/4 (H-134), cDNA Synthesis:Article Title: Autoimmune Regulator (AIRE) Is Expressed in Spermatogenic Cells, and It Altered the Expression of Several Nucleic-Acid-Binding and Cytoskeletal Proteins in Germ Cell 1 Spermatogonial (GC1-spg) Cells Article Snippet: .. Lipofectamine 2000, DMEM, DMEMF12, OptiMEM, FBS, Antibiotic-antimycotic cocktail LGlutamine, 1X MEM vitamins, Nonessential amino acids (Invitrogen, Green Island, CA), StemPro34 SFM base (Invitrogen, Green Island, CA); EcoR1, Sal1, CIP, NEB buffer 3, EcoR1 Buffer, DNA Ligase and Ligase buffer (New England Biolabs, Ipswich, MA); Nucleobond Xtra midi Plus EF kit (Macherey Nagel, Duren, Germany); Gel extraction kit (GE Healthcare, Buckinghamshire, UK); 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), MgCl2, KCl, NaCl, 2,2',2'',2'''-(Ethane-1,2-diyldinitrilo) tetra acetic acid (EDTA), Glycerol, 6 SUPERSCRIPT ® VILO TM cDNA synthesis kit (Invitrogen, Waltham, MA); SYBR green master mix plates, ABI PRISM ® 384 well optical reaction (Applied Bio systems, Waltham, MA), QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany); Protease inhibitor cocktail, Bovine serum albumin (Calbiochem, San Diego, CA); TRI reagent, Glucose, d-Biotin, Insulin, Pyruvic acid sodium salt, dl-Lactic acid, Ascorbic acid, Sodium selenite, Putrescine, Bovine Apotransferrin, Progesterone, β-Estradiol 17-cypionate, 2-Mercaptoethanol (Sigma- Aldrich, Carlsbad, MO); Fetal bovine serum (Hyclone, Logan, UT); SCP-3, Histone H3 (Abcam, Cambridge, United Kingdom); anti-Aire antibody (sc-33188), AIRE-1 (M-300), GFRα-1 (H-70), OCT-3/4 (H-134), Article Title: Autoimmune Regulator ( AIRE ) Is Expressed in Spermatogenic Cells, and It Altered the Expression of Several Nucleic-Acid-Binding and Cytoskeletal Proteins in Germ Cell 1 Spermatogonial (GC1-spg) Cells Article Snippet: .. Lipofectamine 2000, DMEM, DMEMF12, OptiMEM, FBS, antibiotic–antimycotic mixture l -glutamine, 1X MEM vitamins, nonessential amino acids StemPro34 SFM base (Invitrogen, Green Island, CA); EcoR1, Streptomyces albus 1 (Sal1), calf intestinal phosphatase (CIP), New England Biolab (NEB) buffer 3, EcoR1 Buffer, DNA ligase, and ligase buffer (New England Biolabs, Ipswich, MA); Nucleobond Xtra midi Plus EF kit (Macherey Nagel, Duren, Germany); gel extraction kit (GE Healthcare, Buckinghamshire, UK); 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), MgCl 2 , KCl, NaCl, 2,2′,2′′,2′′′-(ethane-1,2-diyldinitrilo) tetra acetic acid (EDTA), glycerol, SUPERSCRIPT ® VILO TM cDNA synthesis kit (Invitrogen, Waltham, MA); SYBR green master mix plates, ABI PRISM ® 384-well optical reaction (Applied Bio systems, Waltham, MA), QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany); protease inhibitor mixture, bovine serum albumin (Calbiochem, San Diego, CA); Total RNA isolation (TRI) reagent, glucose, d-biotin, insulin, pyruvic acid sodium salt, dl-lactic acid, ascorbic acid, sodium selenite, putrescine, bovine Apo-transferrin, progesterone, β-estradiol 17-cypionate, 2-mercaptoethanol (Sigma- Aldrich, Carlsbad, MO); fetal bovine serum (Hyclone, Logan, UT); SCP-3, histone H3 (Abcam, Cambridge, United Kingdom); anti-Aire antibody (sc-33188), AIRE-1 (M-300), GFRα-1 (H-70), OCT-3/4 (H-134), SYBR Green Assay:Article Title: Autoimmune Regulator (AIRE) Is Expressed in Spermatogenic Cells, and It Altered the Expression of Several Nucleic-Acid-Binding and Cytoskeletal Proteins in Germ Cell 1 Spermatogonial (GC1-spg) Cells Article Snippet: .. Lipofectamine 2000, DMEM, DMEMF12, OptiMEM, FBS, Antibiotic-antimycotic cocktail LGlutamine, 1X MEM vitamins, Nonessential amino acids (Invitrogen, Green Island, CA), StemPro34 SFM base (Invitrogen, Green Island, CA); EcoR1, Sal1, CIP, NEB buffer 3, EcoR1 Buffer, DNA Ligase and Ligase buffer (New England Biolabs, Ipswich, MA); Nucleobond Xtra midi Plus EF kit (Macherey Nagel, Duren, Germany); Gel extraction kit (GE Healthcare, Buckinghamshire, UK); 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), MgCl2, KCl, NaCl, 2,2',2'',2'''-(Ethane-1,2-diyldinitrilo) tetra acetic acid (EDTA), Glycerol, 6 SUPERSCRIPT ® VILO TM cDNA synthesis kit (Invitrogen, Waltham, MA); SYBR green master mix plates, ABI PRISM ® 384 well optical reaction (Applied Bio systems, Waltham, MA), QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany); Protease inhibitor cocktail, Bovine serum albumin (Calbiochem, San Diego, CA); TRI reagent, Glucose, d-Biotin, Insulin, Pyruvic acid sodium salt, dl-Lactic acid, Ascorbic acid, Sodium selenite, Putrescine, Bovine Apotransferrin, Progesterone, β-Estradiol 17-cypionate, 2-Mercaptoethanol (Sigma- Aldrich, Carlsbad, MO); Fetal bovine serum (Hyclone, Logan, UT); SCP-3, Histone H3 (Abcam, Cambridge, United Kingdom); anti-Aire antibody (sc-33188), AIRE-1 (M-300), GFRα-1 (H-70), OCT-3/4 (H-134), Article Title: Autoimmune Regulator ( AIRE ) Is Expressed in Spermatogenic Cells, and It Altered the Expression of Several Nucleic-Acid-Binding and Cytoskeletal Proteins in Germ Cell 1 Spermatogonial (GC1-spg) Cells Article Snippet: .. Reagents Lipofectamine 2000, DMEM, DMEMF12, OptiMEM, FBS, antibiotic–antimycotic mixture l -glutamine, 1X MEM vitamins, nonessential amino acids StemPro34 SFM base (Invitrogen, Green Island, CA); EcoR1, Streptomyces albus 1 (Sal1), calf intestinal phosphatase (CIP), New England Biolab (NEB) buffer 3, EcoR1 Buffer, DNA ligase, and ligase buffer (New England Biolabs, Ipswich, MA); Nucleobond Xtra midi Plus EF kit (Macherey Nagel, Duren, Germany); gel extraction kit (GE Healthcare, Buckinghamshire, UK); 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), MgCl 2 , KCl, NaCl, 2,2′,2″,2‴-(ethane-1,2-diyldinitrilo) tetra acetic acid (EDTA), glycerol, SUPERSCRIPT ® VILO TM cDNA synthesis kit (Invitrogen, Waltham, MA); SYBR green master mix plates, ABI PRISM ® 384-well optical reaction (Applied Bio systems, Waltham, MA), QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany); protease inhibitor mixture, bovine serum albumin (Calbiochem, San Diego, CA); Total RNA isolation (TRI) reagent, glucose, d-biotin, insulin, pyruvic acid sodium salt, dl-lactic acid, ascorbic acid, sodium selenite, putrescine, bovine Apo-transferrin, progesterone, β-estradiol 17-cypionate, 2-mercaptoethanol (Sigma- Aldrich, Carlsbad, MO); fetal bovine serum (Hyclone, Logan, UT); SCP-3, histone H3 (Abcam, Cambridge, United Kingdom); anti-Aire antibody (sc-33188), AIRE-1 (M-300), GFRα-1 (H-70), OCT-3/4 (H-134), Article Title: Autoimmune Regulator ( AIRE ) Is Expressed in Spermatogenic Cells, and It Altered the Expression of Several Nucleic-Acid-Binding and Cytoskeletal Proteins in Germ Cell 1 Spermatogonial (GC1-spg) Cells Article Snippet: .. Lipofectamine 2000, DMEM, DMEMF12, OptiMEM, FBS, antibiotic–antimycotic mixture l -glutamine, 1X MEM vitamins, nonessential amino acids StemPro34 SFM base (Invitrogen, Green Island, CA); EcoR1, Streptomyces albus 1 (Sal1), calf intestinal phosphatase (CIP), New England Biolab (NEB) buffer 3, EcoR1 Buffer, DNA ligase, and ligase buffer (New England Biolabs, Ipswich, MA); Nucleobond Xtra midi Plus EF kit (Macherey Nagel, Duren, Germany); gel extraction kit (GE Healthcare, Buckinghamshire, UK); 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), MgCl 2 , KCl, NaCl, 2,2′,2′′,2′′′-(ethane-1,2-diyldinitrilo) tetra acetic acid (EDTA), glycerol, SUPERSCRIPT ® VILO TM cDNA synthesis kit (Invitrogen, Waltham, MA); SYBR green master mix plates, ABI PRISM ® 384-well optical reaction (Applied Bio systems, Waltham, MA), QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany); protease inhibitor mixture, bovine serum albumin (Calbiochem, San Diego, CA); Total RNA isolation (TRI) reagent, glucose, d-biotin, insulin, pyruvic acid sodium salt, dl-lactic acid, ascorbic acid, sodium selenite, putrescine, bovine Apo-transferrin, progesterone, β-estradiol 17-cypionate, 2-mercaptoethanol (Sigma- Aldrich, Carlsbad, MO); fetal bovine serum (Hyclone, Logan, UT); SCP-3, histone H3 (Abcam, Cambridge, United Kingdom); anti-Aire antibody (sc-33188), AIRE-1 (M-300), GFRα-1 (H-70), OCT-3/4 (H-134), Protease Inhibitor:Article Title: Autoimmune Regulator (AIRE) Is Expressed in Spermatogenic Cells, and It Altered the Expression of Several Nucleic-Acid-Binding and Cytoskeletal Proteins in Germ Cell 1 Spermatogonial (GC1-spg) Cells Article Snippet: .. Lipofectamine 2000, DMEM, DMEMF12, OptiMEM, FBS, Antibiotic-antimycotic cocktail LGlutamine, 1X MEM vitamins, Nonessential amino acids (Invitrogen, Green Island, CA), StemPro34 SFM base (Invitrogen, Green Island, CA); EcoR1, Sal1, CIP, NEB buffer 3, EcoR1 Buffer, DNA Ligase and Ligase buffer (New England Biolabs, Ipswich, MA); Nucleobond Xtra midi Plus EF kit (Macherey Nagel, Duren, Germany); Gel extraction kit (GE Healthcare, Buckinghamshire, UK); 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), MgCl2, KCl, NaCl, 2,2',2'',2'''-(Ethane-1,2-diyldinitrilo) tetra acetic acid (EDTA), Glycerol, 6 SUPERSCRIPT ® VILO TM cDNA synthesis kit (Invitrogen, Waltham, MA); SYBR green master mix plates, ABI PRISM ® 384 well optical reaction (Applied Bio systems, Waltham, MA), QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany); Protease inhibitor cocktail, Bovine serum albumin (Calbiochem, San Diego, CA); TRI reagent, Glucose, d-Biotin, Insulin, Pyruvic acid sodium salt, dl-Lactic acid, Ascorbic acid, Sodium selenite, Putrescine, Bovine Apotransferrin, Progesterone, β-Estradiol 17-cypionate, 2-Mercaptoethanol (Sigma- Aldrich, Carlsbad, MO); Fetal bovine serum (Hyclone, Logan, UT); SCP-3, Histone H3 (Abcam, Cambridge, United Kingdom); anti-Aire antibody (sc-33188), AIRE-1 (M-300), GFRα-1 (H-70), OCT-3/4 (H-134), Article Title: Autoimmune Regulator ( AIRE ) Is Expressed in Spermatogenic Cells, and It Altered the Expression of Several Nucleic-Acid-Binding and Cytoskeletal Proteins in Germ Cell 1 Spermatogonial (GC1-spg) Cells Article Snippet: .. Reagents Lipofectamine 2000, DMEM, DMEMF12, OptiMEM, FBS, antibiotic–antimycotic mixture l -glutamine, 1X MEM vitamins, nonessential amino acids StemPro34 SFM base (Invitrogen, Green Island, CA); EcoR1, Streptomyces albus 1 (Sal1), calf intestinal phosphatase (CIP), New England Biolab (NEB) buffer 3, EcoR1 Buffer, DNA ligase, and ligase buffer (New England Biolabs, Ipswich, MA); Nucleobond Xtra midi Plus EF kit (Macherey Nagel, Duren, Germany); gel extraction kit (GE Healthcare, Buckinghamshire, UK); 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), MgCl 2 , KCl, NaCl, 2,2′,2″,2‴-(ethane-1,2-diyldinitrilo) tetra acetic acid (EDTA), glycerol, SUPERSCRIPT ® VILO TM cDNA synthesis kit (Invitrogen, Waltham, MA); SYBR green master mix plates, ABI PRISM ® 384-well optical reaction (Applied Bio systems, Waltham, MA), QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany); protease inhibitor mixture, bovine serum albumin (Calbiochem, San Diego, CA); Total RNA isolation (TRI) reagent, glucose, d-biotin, insulin, pyruvic acid sodium salt, dl-lactic acid, ascorbic acid, sodium selenite, putrescine, bovine Apo-transferrin, progesterone, β-estradiol 17-cypionate, 2-mercaptoethanol (Sigma- Aldrich, Carlsbad, MO); fetal bovine serum (Hyclone, Logan, UT); SCP-3, histone H3 (Abcam, Cambridge, United Kingdom); anti-Aire antibody (sc-33188), AIRE-1 (M-300), GFRα-1 (H-70), OCT-3/4 (H-134), Article Title: Autoimmune Regulator ( AIRE ) Is Expressed in Spermatogenic Cells, and It Altered the Expression of Several Nucleic-Acid-Binding and Cytoskeletal Proteins in Germ Cell 1 Spermatogonial (GC1-spg) Cells Article Snippet: .. Lipofectamine 2000, DMEM, DMEMF12, OptiMEM, FBS, antibiotic–antimycotic mixture l -glutamine, 1X MEM vitamins, nonessential amino acids StemPro34 SFM base (Invitrogen, Green Island, CA); EcoR1, Streptomyces albus 1 (Sal1), calf intestinal phosphatase (CIP), New England Biolab (NEB) buffer 3, EcoR1 Buffer, DNA ligase, and ligase buffer (New England Biolabs, Ipswich, MA); Nucleobond Xtra midi Plus EF kit (Macherey Nagel, Duren, Germany); gel extraction kit (GE Healthcare, Buckinghamshire, UK); 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), MgCl 2 , KCl, NaCl, 2,2′,2′′,2′′′-(ethane-1,2-diyldinitrilo) tetra acetic acid (EDTA), glycerol, SUPERSCRIPT ® VILO TM cDNA synthesis kit (Invitrogen, Waltham, MA); SYBR green master mix plates, ABI PRISM ® 384-well optical reaction (Applied Bio systems, Waltham, MA), QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany); protease inhibitor mixture, bovine serum albumin (Calbiochem, San Diego, CA); Total RNA isolation (TRI) reagent, glucose, d-biotin, insulin, pyruvic acid sodium salt, dl-lactic acid, ascorbic acid, sodium selenite, putrescine, bovine Apo-transferrin, progesterone, β-estradiol 17-cypionate, 2-mercaptoethanol (Sigma- Aldrich, Carlsbad, MO); fetal bovine serum (Hyclone, Logan, UT); SCP-3, histone H3 (Abcam, Cambridge, United Kingdom); anti-Aire antibody (sc-33188), AIRE-1 (M-300), GFRα-1 (H-70), OCT-3/4 (H-134), Cellenone:Article Title: Autoimmune Regulator (AIRE) Is Expressed in Spermatogenic Cells, and It Altered the Expression of Several Nucleic-Acid-Binding and Cytoskeletal Proteins in Germ Cell 1 Spermatogonial (GC1-spg) Cells Article Snippet: .. Lipofectamine 2000, DMEM, DMEMF12, OptiMEM, FBS, Antibiotic-antimycotic cocktail LGlutamine, 1X MEM vitamins, Nonessential amino acids (Invitrogen, Green Island, CA), StemPro34 SFM base (Invitrogen, Green Island, CA); EcoR1, Sal1, CIP, NEB buffer 3, EcoR1 Buffer, DNA Ligase and Ligase buffer (New England Biolabs, Ipswich, MA); Nucleobond Xtra midi Plus EF kit (Macherey Nagel, Duren, Germany); Gel extraction kit (GE Healthcare, Buckinghamshire, UK); 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), MgCl2, KCl, NaCl, 2,2',2'',2'''-(Ethane-1,2-diyldinitrilo) tetra acetic acid (EDTA), Glycerol, 6 SUPERSCRIPT ® VILO TM cDNA synthesis kit (Invitrogen, Waltham, MA); SYBR green master mix plates, ABI PRISM ® 384 well optical reaction (Applied Bio systems, Waltham, MA), QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany); Protease inhibitor cocktail, Bovine serum albumin (Calbiochem, San Diego, CA); TRI reagent, Glucose, d-Biotin, Insulin, Pyruvic acid sodium salt, dl-Lactic acid, Ascorbic acid, Sodium selenite, Putrescine, Bovine Apotransferrin, Progesterone, β-Estradiol 17-cypionate, 2-Mercaptoethanol (Sigma- Aldrich, Carlsbad, MO); Fetal bovine serum (Hyclone, Logan, UT); SCP-3, Histone H3 (Abcam, Cambridge, United Kingdom); anti-Aire antibody (sc-33188), AIRE-1 (M-300), GFRα-1 (H-70), OCT-3/4 (H-134), Article Title: Autoimmune Regulator ( AIRE ) Is Expressed in Spermatogenic Cells, and It Altered the Expression of Several Nucleic-Acid-Binding and Cytoskeletal Proteins in Germ Cell 1 Spermatogonial (GC1-spg) Cells Article Snippet: .. Lipofectamine 2000, DMEM, DMEMF12, OptiMEM, FBS, antibiotic–antimycotic mixture l -glutamine, 1X MEM vitamins, nonessential amino acids StemPro34 SFM base (Invitrogen, Green Island, CA); EcoR1, Streptomyces albus 1 (Sal1), calf intestinal phosphatase (CIP), New England Biolab (NEB) buffer 3, EcoR1 Buffer, DNA ligase, and ligase buffer (New England Biolabs, Ipswich, MA); Nucleobond Xtra midi Plus EF kit (Macherey Nagel, Duren, Germany); gel extraction kit (GE Healthcare, Buckinghamshire, UK); 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), MgCl 2 , KCl, NaCl, 2,2′,2′′,2′′′-(ethane-1,2-diyldinitrilo) tetra acetic acid (EDTA), glycerol, SUPERSCRIPT ® VILO TM cDNA synthesis kit (Invitrogen, Waltham, MA); SYBR green master mix plates, ABI PRISM ® 384-well optical reaction (Applied Bio systems, Waltham, MA), QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany); protease inhibitor mixture, bovine serum albumin (Calbiochem, San Diego, CA); Total RNA isolation (TRI) reagent, glucose, d-biotin, insulin, pyruvic acid sodium salt, dl-lactic acid, ascorbic acid, sodium selenite, putrescine, bovine Apo-transferrin, progesterone, β-estradiol 17-cypionate, 2-mercaptoethanol (Sigma- Aldrich, Carlsbad, MO); fetal bovine serum (Hyclone, Logan, UT); SCP-3, histone H3 (Abcam, Cambridge, United Kingdom); anti-Aire antibody (sc-33188), AIRE-1 (M-300), GFRα-1 (H-70), OCT-3/4 (H-134), Isolation:Article Title: Autoimmune Regulator ( AIRE ) Is Expressed in Spermatogenic Cells, and It Altered the Expression of Several Nucleic-Acid-Binding and Cytoskeletal Proteins in Germ Cell 1 Spermatogonial (GC1-spg) Cells Article Snippet: .. Reagents Lipofectamine 2000, DMEM, DMEMF12, OptiMEM, FBS, antibiotic–antimycotic mixture l -glutamine, 1X MEM vitamins, nonessential amino acids StemPro34 SFM base (Invitrogen, Green Island, CA); EcoR1, Streptomyces albus 1 (Sal1), calf intestinal phosphatase (CIP), New England Biolab (NEB) buffer 3, EcoR1 Buffer, DNA ligase, and ligase buffer (New England Biolabs, Ipswich, MA); Nucleobond Xtra midi Plus EF kit (Macherey Nagel, Duren, Germany); gel extraction kit (GE Healthcare, Buckinghamshire, UK); 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), MgCl 2 , KCl, NaCl, 2,2′,2″,2‴-(ethane-1,2-diyldinitrilo) tetra acetic acid (EDTA), glycerol, SUPERSCRIPT ® VILO TM cDNA synthesis kit (Invitrogen, Waltham, MA); SYBR green master mix plates, ABI PRISM ® 384-well optical reaction (Applied Bio systems, Waltham, MA), QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany); protease inhibitor mixture, bovine serum albumin (Calbiochem, San Diego, CA); Total RNA isolation (TRI) reagent, glucose, d-biotin, insulin, pyruvic acid sodium salt, dl-lactic acid, ascorbic acid, sodium selenite, putrescine, bovine Apo-transferrin, progesterone, β-estradiol 17-cypionate, 2-mercaptoethanol (Sigma- Aldrich, Carlsbad, MO); fetal bovine serum (Hyclone, Logan, UT); SCP-3, histone H3 (Abcam, Cambridge, United Kingdom); anti-Aire antibody (sc-33188), AIRE-1 (M-300), GFRα-1 (H-70), OCT-3/4 (H-134), Article Title: Autoimmune Regulator ( AIRE ) Is Expressed in Spermatogenic Cells, and It Altered the Expression of Several Nucleic-Acid-Binding and Cytoskeletal Proteins in Germ Cell 1 Spermatogonial (GC1-spg) Cells Article Snippet: .. Lipofectamine 2000, DMEM, DMEMF12, OptiMEM, FBS, antibiotic–antimycotic mixture l -glutamine, 1X MEM vitamins, nonessential amino acids StemPro34 SFM base (Invitrogen, Green Island, CA); EcoR1, Streptomyces albus 1 (Sal1), calf intestinal phosphatase (CIP), New England Biolab (NEB) buffer 3, EcoR1 Buffer, DNA ligase, and ligase buffer (New England Biolabs, Ipswich, MA); Nucleobond Xtra midi Plus EF kit (Macherey Nagel, Duren, Germany); gel extraction kit (GE Healthcare, Buckinghamshire, UK); 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), MgCl 2 , KCl, NaCl, 2,2′,2′′,2′′′-(ethane-1,2-diyldinitrilo) tetra acetic acid (EDTA), glycerol, SUPERSCRIPT ® VILO TM cDNA synthesis kit (Invitrogen, Waltham, MA); SYBR green master mix plates, ABI PRISM ® 384-well optical reaction (Applied Bio systems, Waltham, MA), QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany); protease inhibitor mixture, bovine serum albumin (Calbiochem, San Diego, CA); Total RNA isolation (TRI) reagent, glucose, d-biotin, insulin, pyruvic acid sodium salt, dl-lactic acid, ascorbic acid, sodium selenite, putrescine, bovine Apo-transferrin, progesterone, β-estradiol 17-cypionate, 2-mercaptoethanol (Sigma- Aldrich, Carlsbad, MO); fetal bovine serum (Hyclone, Logan, UT); SCP-3, histone H3 (Abcam, Cambridge, United Kingdom); anti-Aire antibody (sc-33188), AIRE-1 (M-300), GFRα-1 (H-70), OCT-3/4 (H-134), Incubation:Article Title: Generation of Haploid Spermatids with Fertilization and Development Capacity from Human Spermatogonial Stem Cells of Cryptorchid Patients Article Snippet: .. The cells were incubated with primary antibodies against UCHL1 (AbD Serotec), GFRA1 (Abcam), SCP3 (Abcam), Article Title: Generation of Haploid Spermatids with Fertilization and Development Capacity from Human Spermatogonial Stem Cells of Cryptorchid Patients Article Snippet: .. The cells were incubated with primary antibodies against UCHL1 (AbD Serotec), GFRA1 (Abcam), SCP3 (Abcam), |
